Info-enriched characterization of cell populations. Morphological data from photographs can include to the richness of flow cytometry facts. For instance, the figure shows an otherwise regular apoptosis assay making use of Annexin V and PI, adding mobile imaging to characterize cells in Every inhabitants to expose morphologically unique characteristics. These insights could not have been obtained from multiplex staining by yourself.
Whether you’re a beginner or an authority, discover our sources to advance your exploration and know-how in flow cytometry.
Merge acoustic concentrating technological know-how with substantial-parameter analysis for adaptable spectral unmixing and standard workflows.
Uncover new insights into your experimental information by visualizing populations utilizing merged fluorescence and morphology facts. Our software program interface wizards permit downsampling, concatenation (merged files) and dimensionality applications which might be constructed to the program for an intuitive, streamlined knowledge Assessment working experience.
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On top of that, it is actually illuminating to determine the amount of doublets slip by traditional singlet gates, and terrific to confirm the status of unforeseen double-positive events in my Assessment."
Optimizing gating tactic. Even strong handbook singlet gating is error-prone and stays a subjective selection stage in Virtually all flow cytometry assays. Imaging may be used to confirm and change gates to include only solitary cells of desire.
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Early signs of this contamination experienced at first been dismissed as debris. Backgating to find out new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also enables you to use morphological attributes to find out interesting subpopulations that would not be evident from flow cytometry knowledge by yourself.
Potentially most importantly, these situations contain cells of clearly diverse phenotypes which may lead to incorrect conclusions pertaining to double favourable gatherings (specifically in exceptional populations).
Seem waves enable precise cell alignment, causing Extraordinary facts good quality and ~10X speedier sample flow premiums.
Application Notice: Automated impression analysis decreases person-to-person variability in flow cytometry gating procedures
Leverage the power of Innovative technology and innovation to accelerate your discoveries. Have faith in inside the Attune Flow Cytometer household to get more info deliver the reliability, speed, and precision that set your get the job done aside.
No clean/no lyse leukocyte Examination with imaging. Cells were being acquired from 24-hour-outdated blood by dilution in 1 mM EDTA ( Pro UX/UI allows users to practice AI models for expanded purposes. (A) The “Teach Design” aspect delivers annotation tools and authentic-time model instruction accuracy.
At the guts of each and every Attune Flow Cytometer lies our groundbreaking acoustic focusing know-how, which makes use of audio waves to meticulously align cells within the flow mobile. This innovative tactic surpasses standard hydrodynamic methods, resulting in more rapidly processing speeds, Excellent facts high-quality, and greater sample throughput.
The Attune Xenith cytometer is often a substantial-parameter spectral analyzer, furnishing both spectral unmixing and conventional flow abilities for extensive and adaptable mobile Investigation.
Agilent Value Promise guarantees you no less than 10 years of instrument use with the date you purchase. Normally, We'll credit you Along with the residual value of the program towards an upgraded product.
What seemed to be a homogeneous inhabitants is in fact much more assorted—an insight that ought to be regarded when interpreting final results.